Application of HPLC to the assay of enzymatic activities.

This chapter describes the anatomy of an enzyme assay, focusing on the significance of separation and detection in the assay procedure. A classification of the methods used in the assay of enzymatic activities is developed, using the separation step as the criterion for the grouping. Having placed the high performance liquid chromatography (HPLC) method within this classification, we then examine the question of when to use it and discuss some strategies developed for its use. The chapter also identifies and comments on the parts of the enzyme assay that will be affected by the selection of HPLC as the method of analysis.